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il 17c  (R&D Systems)


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    Structured Review

    R&D Systems il 17c
    Il 17c, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+17c/Recombinant+Human+IL-17C+Protein/10__64943_slash_ljmas__2026__04133-89-22-23
    Average 94 stars, based on 11 article reviews
    il 17c - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Il-17 Family Cytokines in Psoriasis: Radioactive Splendor Dual Role in Inflammation and Tissue Re Modeling
    Article Snippet: Article history: Psoriasis is an immunologically mediated distressed skin disease of chronic tendency that is correlated to the over proliferation of keratinocytes and chronic inflammation.. IL-17A is a known therapeutic target but IL-17 cytokine family (as IL-17F, IL17C and IL-17E) can also mediate the inflammation and structural reorganization of psoriatic skin.To study the dual function of IL-17 family cytokines to regulate inflammatory reactions and tissue remodeling in psoriasis, and their relationship with clinical severity.. The case-control study design was used, and 100 patients with moderateto severe plaque psoriasis and 100 healthy controls matched according to 12 age-matched design factors were included.

    Article Title: Th17-Derived Cytokines Synergistically Enhance IL-17C Production by the Colonic Epithelium.
    Article Snippet: .. Recombinant human TNF-a, IL-17A, IL-22, and IL-17C were purchased from R&D Systems. ..

    Article Title: Inflammasome activation has an important role in the development of spontaneous colitis.
    Article Snippet: .. Recombinant human (rh) IL-17A, IL-17C, IL-22, TNF-a, transforming growth factor-b, granulocyte-macrophages colonystimulating factor, and IL-4 were purchased from R&D Systems (Minneapolis, MN), IL-12, IL-23, and IL-6 from eBioscience (Frankfurt, Germany). rhIL-17C was additionally obtained from eBioscience and Abnova (Heidelberg, Germany). ..

    Article Title: Intestinal neuroendocrine cells and goblet cells are mediators of IL-17A-amplified epithelial IL-17C production in human inflammatory bowel disease.
    Article Snippet: .. Recombinant human (rh) IL-17A, IL-17C, IL-22, TNF-a, transforming growth factor-b, granulocyte-macrophages colonystimulating factor, and IL-4 were purchased from R&D Systems (Minneapolis, MN), IL-12, IL-23, and IL-6 from eBioscience (Frankfurt, Germany). rhIL-17C was additionally obtained from eBioscience and Abnova (Heidelberg, Germany). ..

    Article Title: Hyperinsulinemia enhances interleukin-17-induced inflammation to promote prostate cancer development in obese mice through inhibiting glycogen synthase kinase 3-mediated phosphorylation and degradation of interleukin-17 receptor
    Article Snippet: .. Human HeLa and 293-IL-17RA stable cells were treated with 2 μM AZD5363 (Selleck Chemicals, Inc.), 50 ng/ml insulin (Sigma Aldrich), and/or 20 ng/ml recombinant human IL-17A (R & D Systems) for 2 h. Mouse embryonic fibroblasts (MEFs) with wild-type Gsk3β +/+ or knockout Gsk3β −/− were treated with 20 ng/ml recombinant mouse IL-17A, IL-17F, IL-17C, or IL-17E (R & D Systems) for 2 h. RNA was isolated for qRT-PCR analysis as described previously [ ]. .. Human prostate and skin tissue samples were provided by Ochsner Clinic Foundation (for prostate tissues) and the Affiliated Hospital of Guangdong Medical College (for skin tissues) with approval of the Institutional Review Boards of Ochsner Clinic Foundation and Guangdong Medical College, respectively.

    Cell Culture:

    Article Title: Calcipotriol and betamethasone dipropionate exert additive inhibitory effects on the cytokine expression of inflammatory dendritic cell-Th17 cell axis in psoriasis.
    Article Snippet: .. After 48 h of treatment, cell culture supernatants were collected for protein analysis of IL-20 (Human IL-20 Quantikine) and IL-17C (Human IL-17C DuoSet) using ELISA kits from R&D Systems and of IL-8 and IL-6 using Human ProInflammatory-4 II Ultra-Sensitive Kit from Meso Scale Diagnostics. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Calcipotriol and betamethasone dipropionate exert additive inhibitory effects on the cytokine expression of inflammatory dendritic cell-Th17 cell axis in psoriasis.
    Article Snippet: .. After 48 h of treatment, cell culture supernatants were collected for protein analysis of IL-20 (Human IL-20 Quantikine) and IL-17C (Human IL-17C DuoSet) using ELISA kits from R&D Systems and of IL-8 and IL-6 using Human ProInflammatory-4 II Ultra-Sensitive Kit from Meso Scale Diagnostics. ..

    Knock-Out:

    Article Title: Hyperinsulinemia enhances interleukin-17-induced inflammation to promote prostate cancer development in obese mice through inhibiting glycogen synthase kinase 3-mediated phosphorylation and degradation of interleukin-17 receptor
    Article Snippet: .. Human HeLa and 293-IL-17RA stable cells were treated with 2 μM AZD5363 (Selleck Chemicals, Inc.), 50 ng/ml insulin (Sigma Aldrich), and/or 20 ng/ml recombinant human IL-17A (R & D Systems) for 2 h. Mouse embryonic fibroblasts (MEFs) with wild-type Gsk3β +/+ or knockout Gsk3β −/− were treated with 20 ng/ml recombinant mouse IL-17A, IL-17F, IL-17C, or IL-17E (R & D Systems) for 2 h. RNA was isolated for qRT-PCR analysis as described previously [ ]. .. Human prostate and skin tissue samples were provided by Ochsner Clinic Foundation (for prostate tissues) and the Affiliated Hospital of Guangdong Medical College (for skin tissues) with approval of the Institutional Review Boards of Ochsner Clinic Foundation and Guangdong Medical College, respectively.

    Isolation:

    Article Title: Hyperinsulinemia enhances interleukin-17-induced inflammation to promote prostate cancer development in obese mice through inhibiting glycogen synthase kinase 3-mediated phosphorylation and degradation of interleukin-17 receptor
    Article Snippet: .. Human HeLa and 293-IL-17RA stable cells were treated with 2 μM AZD5363 (Selleck Chemicals, Inc.), 50 ng/ml insulin (Sigma Aldrich), and/or 20 ng/ml recombinant human IL-17A (R & D Systems) for 2 h. Mouse embryonic fibroblasts (MEFs) with wild-type Gsk3β +/+ or knockout Gsk3β −/− were treated with 20 ng/ml recombinant mouse IL-17A, IL-17F, IL-17C, or IL-17E (R & D Systems) for 2 h. RNA was isolated for qRT-PCR analysis as described previously [ ]. .. Human prostate and skin tissue samples were provided by Ochsner Clinic Foundation (for prostate tissues) and the Affiliated Hospital of Guangdong Medical College (for skin tissues) with approval of the Institutional Review Boards of Ochsner Clinic Foundation and Guangdong Medical College, respectively.

    Quantitative RT-PCR:

    Article Title: Hyperinsulinemia enhances interleukin-17-induced inflammation to promote prostate cancer development in obese mice through inhibiting glycogen synthase kinase 3-mediated phosphorylation and degradation of interleukin-17 receptor
    Article Snippet: .. Human HeLa and 293-IL-17RA stable cells were treated with 2 μM AZD5363 (Selleck Chemicals, Inc.), 50 ng/ml insulin (Sigma Aldrich), and/or 20 ng/ml recombinant human IL-17A (R & D Systems) for 2 h. Mouse embryonic fibroblasts (MEFs) with wild-type Gsk3β +/+ or knockout Gsk3β −/− were treated with 20 ng/ml recombinant mouse IL-17A, IL-17F, IL-17C, or IL-17E (R & D Systems) for 2 h. RNA was isolated for qRT-PCR analysis as described previously [ ]. .. Human prostate and skin tissue samples were provided by Ochsner Clinic Foundation (for prostate tissues) and the Affiliated Hospital of Guangdong Medical College (for skin tissues) with approval of the Institutional Review Boards of Ochsner Clinic Foundation and Guangdong Medical College, respectively.

    Incubation:

    Article Title: Suppression of TCF4 promotes a ZC3H12A-mediated self-sustaining inflammatory feedback cycle involving IL-17RA/IL-17RE epidermal signaling
    Article Snippet: .. Slides were treated with 3% H 2 O 2 (5 minutes), blocked with 10% goat serum (30 minutes), and incubated with primary antibodies against TCF4 (HPA025958-100UL, Sigma-Aldrich), IL-17C (AF1234, R&D Systems), and rabbit IgG isotype control (NI01, Sigma-Aldrich) overnight at 4°C. ..

    Control:

    Article Title: Suppression of TCF4 promotes a ZC3H12A-mediated self-sustaining inflammatory feedback cycle involving IL-17RA/IL-17RE epidermal signaling
    Article Snippet: .. Slides were treated with 3% H 2 O 2 (5 minutes), blocked with 10% goat serum (30 minutes), and incubated with primary antibodies against TCF4 (HPA025958-100UL, Sigma-Aldrich), IL-17C (AF1234, R&D Systems), and rabbit IgG isotype control (NI01, Sigma-Aldrich) overnight at 4°C. ..



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    Effect of <t>IL-17C</t> on malignant transformation in IL-17RE knockdown and overexpressed endometriotic cells (A) qPCR and western blot assay were used to measure the transfection efficiency and IL-17RE expression of si-IL-17RE in 11Z and 12Z. (B) Immunofluorescence staining reveals the effect <t>of</t> <t>rhIL-17C</t> on the expression of PCNA in IL-17RE knockdown 11Z and 12Z. (C–E) (C) Colony formation, (D) adhesion, and (E) migration and invasion were detected in parallel 11Z and 12Z cultures. (F and G) Western blot analysis was used to detect the expression of EMT-related and PI3K pathway key proteins in IL-17RE knockdown cells with or without rhIL-17C for 24 h. (H–J) (H) Immunohistological staining of PCNA, (I) colony formation, and (J) migration and invasion was detected in IL-17RE-overexpressing 11Z and 12Z with and without MOR106 treatment for 24 h. (K) Western blot analysis was used to detect the expression of EMT-related and (L) PI3K pathway key proteins in 11Z and 12Z with and without MOR106 treatment. n = 3 biological replicates. Data are represented as mean ± SEM. ∗ p < 0.05 and ∗∗ p < 0.01 for the indicated comparisons. Differences between groups were assessed using one-way ANOVA with Tukey’s post-hoc test. ns, not significant. Scale bars, 100 μm.
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    Effect of <t>IL-17C</t> on malignant transformation in IL-17RE knockdown and overexpressed endometriotic cells (A) qPCR and western blot assay were used to measure the transfection efficiency and IL-17RE expression of si-IL-17RE in 11Z and 12Z. (B) Immunofluorescence staining reveals the effect <t>of</t> <t>rhIL-17C</t> on the expression of PCNA in IL-17RE knockdown 11Z and 12Z. (C–E) (C) Colony formation, (D) adhesion, and (E) migration and invasion were detected in parallel 11Z and 12Z cultures. (F and G) Western blot analysis was used to detect the expression of EMT-related and PI3K pathway key proteins in IL-17RE knockdown cells with or without rhIL-17C for 24 h. (H–J) (H) Immunohistological staining of PCNA, (I) colony formation, and (J) migration and invasion was detected in IL-17RE-overexpressing 11Z and 12Z with and without MOR106 treatment for 24 h. (K) Western blot analysis was used to detect the expression of EMT-related and (L) PI3K pathway key proteins in 11Z and 12Z with and without MOR106 treatment. n = 3 biological replicates. Data are represented as mean ± SEM. ∗ p < 0.05 and ∗∗ p < 0.01 for the indicated comparisons. Differences between groups were assessed using one-way ANOVA with Tukey’s post-hoc test. ns, not significant. Scale bars, 100 μm.
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    Image Search Results


    Effect of IL-17C on malignant transformation in IL-17RE knockdown and overexpressed endometriotic cells (A) qPCR and western blot assay were used to measure the transfection efficiency and IL-17RE expression of si-IL-17RE in 11Z and 12Z. (B) Immunofluorescence staining reveals the effect of rhIL-17C on the expression of PCNA in IL-17RE knockdown 11Z and 12Z. (C–E) (C) Colony formation, (D) adhesion, and (E) migration and invasion were detected in parallel 11Z and 12Z cultures. (F and G) Western blot analysis was used to detect the expression of EMT-related and PI3K pathway key proteins in IL-17RE knockdown cells with or without rhIL-17C for 24 h. (H–J) (H) Immunohistological staining of PCNA, (I) colony formation, and (J) migration and invasion was detected in IL-17RE-overexpressing 11Z and 12Z with and without MOR106 treatment for 24 h. (K) Western blot analysis was used to detect the expression of EMT-related and (L) PI3K pathway key proteins in 11Z and 12Z with and without MOR106 treatment. n = 3 biological replicates. Data are represented as mean ± SEM. ∗ p < 0.05 and ∗∗ p < 0.01 for the indicated comparisons. Differences between groups were assessed using one-way ANOVA with Tukey’s post-hoc test. ns, not significant. Scale bars, 100 μm.

    Journal: Cell Reports Medicine

    Article Title: Therapeutic targeting of interleukin-17C signaling in carcinogenesis of endometriosis

    doi: 10.1016/j.xcrm.2025.102464

    Figure Lengend Snippet: Effect of IL-17C on malignant transformation in IL-17RE knockdown and overexpressed endometriotic cells (A) qPCR and western blot assay were used to measure the transfection efficiency and IL-17RE expression of si-IL-17RE in 11Z and 12Z. (B) Immunofluorescence staining reveals the effect of rhIL-17C on the expression of PCNA in IL-17RE knockdown 11Z and 12Z. (C–E) (C) Colony formation, (D) adhesion, and (E) migration and invasion were detected in parallel 11Z and 12Z cultures. (F and G) Western blot analysis was used to detect the expression of EMT-related and PI3K pathway key proteins in IL-17RE knockdown cells with or without rhIL-17C for 24 h. (H–J) (H) Immunohistological staining of PCNA, (I) colony formation, and (J) migration and invasion was detected in IL-17RE-overexpressing 11Z and 12Z with and without MOR106 treatment for 24 h. (K) Western blot analysis was used to detect the expression of EMT-related and (L) PI3K pathway key proteins in 11Z and 12Z with and without MOR106 treatment. n = 3 biological replicates. Data are represented as mean ± SEM. ∗ p < 0.05 and ∗∗ p < 0.01 for the indicated comparisons. Differences between groups were assessed using one-way ANOVA with Tukey’s post-hoc test. ns, not significant. Scale bars, 100 μm.

    Article Snippet: Moreover, the effects of rhIL-17C (1234-IL-025, R&D Systems), anti-IL-17RE (NEUT-1237CQ, Creative biolabs, USA), MOR106 (BR2010231, Shanghai bioleaper biotechnology Co. Ltd., China) and LY294002 (HY-10108, MCE) were evaluated on cells respectively.

    Techniques: Transformation Assay, Knockdown, Western Blot, Transfection, Expressing, Immunofluorescence, Staining, Migration